Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Tags: His tag C-Terminus, proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
-
Product name
Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) -
Biological activity
The specific activity of ab184883 was determined to be 900 nmol/min/mg.
-
Purity
> 80 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Tags
His tag C-Terminus , proprietary tag N-Terminus -
Additional sequence information
Recombinant full length AMPK alpha 1 (aa 1-559; UniProt Q13131) + AMPK beta 2 (aa 1-272; UniProt O43741) + AMPK gamma 2 (aa 1-525; UniProt Q9UGJ0-3). MWt 92, 62, and 105 kDa.
-
Preparation and Storage
-
Applications
Functional Studies
SDS-PAGE
Western blot
-
Form
Liquid -
Additional notes
AMPK alpha 1 (UniProt Q13131)
MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQAMPK beta 2 (UniProt O43741)
MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPIAMPK gamma 2 (UniProt Q9UGJ0-3)
MPLLDGDLEGSGKHSSRKVDSPFGPGSPSKGFFSRGPQPRPSSPMSAPVRPKTSPGSPKTVFPFSYQESPPRSPRRMSFSGIFRSSSKESSPNSNPATSPGGIRFFSRSRKTSGLSSSPSTPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQSPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETEab204881 (Acetyl Coenzyme A Carboxylase peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Images
-
Functional Studies - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)The specific activity of AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 (ab184883) was determined to be 908 nmol/min/mg as per activity assay protocol
-
SDS-PAGE - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)SDS PAGE analysis of ab184883 -
Functional Studies - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)Kinase Assay demonstrating specific activity of ab184883.
-
SDS-PAGE - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)SDS-PAGE analysis of ab184883.
