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Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 80% Densitometry
  • Active: Yes
  • Tags: His tag C-Terminus, proprietary tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, WB

Description

  • Product name

    Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active)

  • Biological activity

    The specific activity of ab184883 was determined to be 900 nmol/min/mg.

  • Purity

    > 80 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Tags

      His tag C-Terminus , proprietary tag N-Terminus

    • Additional sequence information

      Recombinant full length AMPK alpha 1 (aa 1-559; UniProt Q13131) + AMPK beta 2 (aa 1-272; UniProt O43741) + AMPK gamma 2 (aa 1-525; UniProt Q9UGJ0-3). MWt 92, 62, and 105 kDa.

Preparation and Storage

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

    AMPK alpha 1 (UniProt Q13131)
    MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQ

    AMPK beta 2 (UniProt O43741)
    MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI

    AMPK gamma 2 (UniProt Q9UGJ0-3)
    MPLLDGDLEGSGKHSSRKVDSPFGPGSPSKGFFSRGPQPRPSSPMSAPVRPKTSPGSPKTVFPFSYQESPPRSPRRMSFSGIFRSSSKESSPNSNPATSPGGIRFFSRSRKTSGLSSSPSTPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQSPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE

    ab204881 (Acetyl Coenzyme A Carboxylase peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Images

  • Functional Studies - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)
    Functional Studies - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)
    The specific activity of AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 (ab184883) was determined to be 908 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)
    SDS-PAGE - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)
    SDS PAGE analysis of ab184883
  • Functional Studies - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)
    Functional Studies - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)

    Kinase Assay demonstrating specific activity of ab184883.

  • SDS-PAGE - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)
    SDS-PAGE - Recombinant human AMPK alpha 1 + AMPK beta 2 +AMPK gamma 2 protein (Active) (ab184883)

    SDS-PAGE analysis of ab184883.

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