Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant S. japonicum GST protein (Active) (ab235135)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant S. japonicum GST protein (Active)
    See all GST proteins and peptides

  • Biological activity

    Specific activity is > 30 units/mg, and is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.

  • Purity

    > 90 % SDS-PAGE.
    ab235135 was purified using conventional chromatography techniques.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P08515

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Schistosoma japonicum

    • Sequence

      MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPK

    • Predicted molecular weight

      25 kDa

    • Amino acids

      1 to 218

    • Additional sequence information

      AAB59203.

    • Specifications

    Our Abpromise guarantee covers the use of ab235135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.4
      Constituents: PBS, 10% Glycerol (glycerin, glycerine)

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Glutathione S Transferase
      • Glutathione S-transferase class-mu 26 kDa isozyme
      • GST
      • Sj26 antigen
      • SjGST
      see all

    • Relevance

      GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Images

  • SDS-PAGE - Recombinant <em>S. japonicum</em> GST protein (Active) (ab235135)
    SDS-PAGE - Recombinant S. japonicum GST protein (Active) (ab235135)

    15% SDS-PAGE analysis of 3 µg ab235135.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab235135? Please let us know so that we can cite the reference in this datasheet.

ab235135 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: PBS, 10% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • SDS-PAGE - Recombinant S. japonicum GST protein (Active) (ab235135)
    SDS-PAGE - Recombinant S. japonicum GST protein (Active) (ab235135)

    15% SDS-PAGE analysis of 3 µg ab235135.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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