Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 03, 2021

Anti-Prothrombin antibody [EPR20131] (ab208590)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20131] to Prothrombin
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat

Product name

Anti-Prothrombin antibody [EPR20131]
See all Prothrombin primary antibodies
Description

Rabbit monoclonal [EPR20131] to Prothrombin
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

WB
Mouse

Rat

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat liver lysates; Mouse and rat plasma. IHC-P: Mouse liver and kidney tissues; Rat liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse Rat
WB	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20131
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] (ab208590)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse liver was observed (PMID: 1705822). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] (ab208590)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on plasma and endothelial cells of human kidney was observed (PMID: 16410745). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Prothrombin antibody [EPR20131] (ab208590)

All lanes : Anti-Prothrombin antibody [EPR20131] (ab208590) at 1/1000 dilution

Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 70 kDa
Observed band size: 72 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Prothrombin antibody [EPR20131] (ab208590)

All lanes : Anti-Prothrombin antibody [EPR20131] (ab208590) at 1/1000 dilution

Lane 1 : Mouse plasma
Lane 2 : Rat plasma

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 70 kDa
Observed band size: 33,48,72 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Prothrombin is proteolytically cleaved into different fragments. The fragment profile is consistent with the literature (PMID: 17637839; PMID: 21131592; PMID: 16734589).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prothrombin antibody [EPR20131] (ab208590)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Prothrombin with ab208590 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat liver was observed (PMID: 1705822). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Prothrombin antibody [EPR20131] (ab208590)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com