Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prothrombin antibody [EPR20159] (ab208589)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20159] to Prothrombin
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Prothrombin antibody [EPR20159]
    See all Prothrombin primary antibodies

  • Description

    Rabbit monoclonal [EPR20159] to Prothrombin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma and fetal liver lysate; HepG2 whole cell lysate. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20159

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Prothrombin antibody [EPR20159] (ab208589)
    Western blot - Anti-Prothrombin antibody [EPR20159] (ab208589)
    All lanes : Anti-Prothrombin antibody [EPR20159] (ab208589) at 1/1000 dilution

    Lane 1 : Human plasma at 20 µg
    Lane 2 : Human fetal liver lysate at 20 µg
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 70 kDa
    Observed band size: 33,52,72 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Prothrombin is proteolytically cleaved into different fragments. The fragments profile is consistent with what has been described in the literature (PMID: 17637839; PMID: 21131592; PMID: 16734589).

  • Immunocytochemistry/ Immunofluorescence - Anti-Prothrombin antibody [EPR20159] (ab208589)
    Immunocytochemistry/ Immunofluorescence - Anti-Prothrombin antibody [EPR20159] (ab208589)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Prothrombin with ab208589 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow Cytometry - Anti-Prothrombin antibody [EPR20159] (ab208589)
    Flow Cytometry - Anti-Prothrombin antibody [EPR20159] (ab208589)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Prothrombin with ab208589 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Prothrombin antibody [EPR20159] (ab208589)
    Anti-Prothrombin antibody [EPR20159] (ab208589)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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