Recombinant cynomolgus monkey Glypican 3 protein (Active) (ab220585)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 85% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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SDS-PAGE - Recombinant cynomolgus monkey Glypican 3 protein (Active) (ab220585)Cynomolgus Glypican 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
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Functional Studies - Recombinant cynomolgus monkey Glypican 3 protein (Active) (ab220585)Immobilized Cynomolgus Glypican 3, His Tag at 0.5 µg/mL (100 µL/well) can bind Monoclonal Anti-Human GPC3 Antibody, Human IgG1 with a linear range of 0.8-13 ng/mL. -
Functional Studies - Recombinant cynomolgus monkey Glypican 3 protein (Active) (ab220585)Immobilized Human FGF basic, Tag Free at 0.5 µg/mL (100 µL/well) can bind Cynomolgus Glypican 3, His Tag with a linear range of 1-20 ng/mL. -
SDS-PAGE - Recombinant cynomolgus monkey Glypican 3 protein (Active) (ab220585)SDS-PAGE analysis of reduced ab220585 stained overnight with Coomassie Blue.
The protein migrates as 66-150 kDa, 40 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
