Rat MCP1 Matched Antibody Pair Kit (ab221434)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Rat
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Rat MCP1 Matched Antibody Pair Kit
See all MCP1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.5 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Rat -
Product overview
Rat MCP1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant rat MCP1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219045. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Rat MCP1 Capture Antibody 2 x 50µg 1 x 50µg Rat MCP1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Rat MCP1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 2
- CCL2
- CCL2_HUMAN
see all -
Database links
- Entrez Gene: 24770 Rat
- SwissProt: P14844 Rat
- Unigene: 4772 Rat
Images
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Rat MCP1 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Rat MCP1 Matched Antibody Pair Kit (ab221434)To learn more about the advantages of recombinant antibodies see here.
