Rat IL-17A Matched Antibody Pair Kit (ab218187)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Rat
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Rat IL-17A Matched Antibody Pair Kit
See all IL-17A kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.28 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Rat -
Product overview
Rat IL-17A Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant rat IL-17A.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214028. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Rat IL-17A Capture Antibody 2 x 50µg 1 x 50µg Rat IL-17A Detector Antibody 2 x 12.5µg 1 x 12.5µg Rat IL-17A Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CTLA 8
- CTLA-8
- CTLA8
see all -
Database links
- Entrez Gene: 301289 Rat
- SwissProt: Q61453 Rat
Images
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Rat IL-17A standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Rat IL-17A Matched Antibody Pair Kit (ab218187)To learn more about the advantages of recombinant antibodies see here.
