Rat IL-17A ELISA Kit (ab119536)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 1.6 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-17A ELISA Kit
See all IL-17A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 7 8.5% Inter-assay Sample n Mean SD CV% Overall 7 7.6% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
1.6 pg/ml - 100 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92% - 112% Serum 34% - 44% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s IL-17A Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Rat IL-17 (Interleukin-17)concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
IL-17A specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-17A detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17A captured on the plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-rat IL-17A monoclonal antibody 1 x 70µl Microplate coated with monoclonal antibody to rat IL-17A (12 x 8 wells) 1 unit Rat IL-17A Standard Lyophilized (200 pg/mL on reconstitution) 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CTLA 8
- CTLA-8
- CTLA8
see all -
Database links
- Entrez Gene: 301289 Rat
- SwissProt: Q61453 Rat
Images
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Typical Standard Curve
Representative Standard Curve using ab119536.
