Anti-IL-4 antibody [C1] (ab239508)
![Anti-IL-4 antibody [C1] (ab239508)](https://www.abcam.com/ps/products/239/ab239508/Images/ab239508-318003-anti-il-4-antibody-c1-immunohistochemistry.jpg "Anti-IL-4 antibody [C1] (ab239508)")
Key features and details
- Mouse monoclonal [C1] to IL-4
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG2b
Overview
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Product name
Anti-IL-4 antibody [C1]
See all IL-4 primary antibodies -
Description
Mouse monoclonal [C1] to IL-4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanPig
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human IL-4 aa 1 to the C-terminus. N-terminal His-tag (Expressed in E.coli).
Database link: P05112 -
Positive control
- IHC-P: Human pancreas, prostate gland and breast cancer tissues. WB: Pig spleen lysate; Recombinant human IL4 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4 antibody [C1] (ab239508)
Paraffin-embedded human pancreas tissue stained for IL4 using ab239508 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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![Western blot - Anti-IL-4 antibody [C1] (ab239508)](https://www.abcam.com/ps/products/239/ab239508/Images/ab239508-317999-anti-il-4-antibody-c1-western-blot.jpg)
Western blot - Anti-IL-4 antibody [C1] (ab239508)Anti-IL-4 antibody [C1] (ab239508) at 3 µg/ml + Pig spleen lysate
Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 17 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4 antibody [C1] (ab239508)](https://www.abcam.com/ps/products/239/ab239508/Images/ab239508-318002-anti-il-4-antibody-c1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4 antibody [C1] (ab239508)Paraffin-embedded human prostate gland tissue stained for IL4 using ab239508 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4 antibody [C1] (ab239508)](https://www.abcam.com/ps/products/239/ab239508/Images/ab239508-318001-anti-il-4-antibody-c1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4 antibody [C1] (ab239508)Paraffin-embedded human breast cancer tissue stained for IL4 using ab239508 at 30 µg/ml in immunohistochemical analysis. DAB staining.
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![Western blot - Anti-IL-4 antibody [C1] (ab239508)](https://www.abcam.com/ps/products/239/ab239508/Images/ab239508-318000-anti-il-4-antibody-c1-western-blot.jpg)
Western blot - Anti-IL-4 antibody [C1] (ab239508)Anti-IL-4 antibody [C1] (ab239508) at 3 µg/ml + Recombinant human IL4 protein
Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 17 kDa
