Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)
![Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)](https://www.abcam.com/ps/products/35/ab35768/Images/ab35768-48433-rat-igm-kappa-monoclonal-rtk2118-isotype-control-flow-cytometry.jpg "Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)")
Overview
-
Product name
Rat IgM, kappa monoclonal [RTK2118] - Isotype Control
See all Rat isotype controls -
Tested applications
Suitable for: Flow Cyt, WB, IP, ICC/IF, IHC-Fr, IHC-Pmore details -
Immunogen
Chemical/ Small Molecule corresponding to Rat. Trinitrophenol + KLH
-
General notes
Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Purified IgM -
Isotype control notes
Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies. -
Clonality
Monoclonal -
Clone number
RTK2118 -
Isotype
IgM -
Light chain type
kappa -
Research areas
-
Alternative names
- Rat Isotype Control
Images
-
Flow Cytometry - Rat IgM, kappa monoclonal [RTK2118] - Isotype Control (ab35768)
Overlay histogram showing RAMOS cells stained with ab19795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19795, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat kappa monoclonal [RTK2118] (ab35768, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
