Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
![Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)](https://www.abcam.com/ps/products/18/ab18450/Images/ab18450-28038-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-low-endotoxin-azide-free-flow-cytometry.jpg "Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)")
Overview
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Product name
Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control
See all Rat isotype controls -
Conjugation notes
Please note that this antibody is unconjugated. -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Frmore details -
Immunogen
Trinitrophenol + KLH
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General notes
ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS
0.2 µm filter sterilized -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The immunoglobulin was purified by affinity chromatography. -
Isotype control notes
ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. -
Clonality
Monoclonal -
Clone number
RTK2758 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
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Alternative names
- Rat Isotype Control
Images
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Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.