Anti-IL-10 antibody [JES5-2A5] (ab189392)
Key features and details
- Rat monoclonal [JES5-2A5] to IL-10
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-IL-10 antibody [JES5-2A5]
See all IL-10 primary antibodies -
Description
Rat monoclonal [JES5-2A5] to IL-10 -
Host species
Rat -
Specificity
ab189392 recognizes mouse IL-10. IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse -
Immunogen
Recombinant fragment corresponding to Mouse IL-10.
Database link: P18893 -
Positive control
- Mouse small intestine and spleen tissues. Recombinant mouse IL-10.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JES5-2A5 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, labeling IL-10 using ab189392 at 10 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, labeling IL-10 using ab189392 at 10 µg/ml.
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Anti-IL-10 antibody [JES5-2A5] (ab189392) at 1/1000 dilution + recombinant mouse IL-10
Secondary
F(ab)2 rabbit anti-rat IgG:HRP
Developed using the ECL technique.
Predicted band size: 21 kDa