Rat Beta-2-Microglobulin ELISA Kit (ab264619)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 268 pg/ml
- Range: 439 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Beta-2-Microglobulin ELISA Kit
See all beta 2 Microglobulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 2.1% -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
268 pg/ml -
Range
439 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 115 110% - 123% Urine 119 116% - 123% Serum 115 111% - 118% Cell culture media 99 93% - 104% Hep Plasma 111 106% - 114% EDTA Plasma 118 117% - 119% Cit plasma 109 107% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat Beta-2-Microglobulin ELISA Kit (ab264619) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta-2-Microglobulin protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Beta-2-Microglobulin with 268 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Beta-2-Microglobulin is the light chain of the class I major histocompatibility complex (MHC); it forms heterodimers with classical (HLA-A, -B, -C) and non-classical (HLA-E, -F, -G) class I MHC heavy chains and with class I non-MHC heavy chains (CD1). Beta-2-Microglobulin is involved in the presentation of peptide antigens to the immune system. Beta-2-Microglobulin can dissociate from the MHC and circulate in complex with alpha 2-Macroglobulin.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat Beta-2-Microglobulin Capture Antibody 1 x 600µl 10X Rat Beta-2-Microglobulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 5BR 1 x 6ml Denaturant 1 x 500µl Plate Seals 1 unit Rat Beta-2-Microglobulin Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients. -
Cellular localization
Secreted. Detected in serum and urine. - Information by UniProt
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Alternative names
- B2M
- B2MG_HUMAN
- Beta 2 microglobin
see all -
Database links
- Entrez Gene: 24223 Rat
- SwissProt: P07151 Rat
- Unigene: 1868 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat Beta-2-Microglobulin standard curve in Sample Diluent NS + Enhancer.The Beta-2-Microglobulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Beta-2-Microglobulin in rat serum and plasma samples.The concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:3,000, plasma (citrate) 1:1,500, plasma (heparin) 1:2,250, and plasma (EDTA) 1:2,250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 12.23 µg/mL in neat serum, 7.025 µg/mL in neat plasma (citrate), 8.710 µg/mL in neat plasma (heparin), and 7.155 µg/mL in neat plasma (EDTA).
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Interpolated concentrations of native Beta-2-Microglobulin in rat urine and cell culture supernatant samples.The concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:150 and lung cell culture supernatant 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 491.5 ng/mL in neat rat urine and 434.3 ng/mL in neat rat lung cell culture supernatant.
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Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (ab264619)To learn more about the advantages of recombinant antibodies see here.
