Histamine Assay Kit (Colorimetric) (ab235630)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
Overview
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Product name
Histamine Assay Kit (Colorimetric)
See all Histamine kits -
Detection method
Colorimetric -
Assay type
Quantitative -
Product overview
Histamine Assay Kit (Colorimetric) (ab235630) can be used to identify dangerous levels of histamine in various fish and meat products, as well as other fermented food and sauces/beverages. While the body is accustomed to exposure to low levels (30-50 ppm, or mg/L) of histamine, ingestion of food containing levels higher than several hundred parts per million can lead to adverse effects.
With this kit histamine can be detected in samples at concentrations as low as 10 μM, or 10 μg/mL (10 ppm). This kit is very specific and does not interact with other bioamines.
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Notes
Histamine is a biogenic amine with considerable biological relevance. As a signaling molecule histamine plays manifold roles in the body, ranging from local immune responses to neurotransmission. Histamine is also frequently encountered in food, particularly fish and fermented food products such as sauerkraut and aged cheeses. This is because some bacteria generate histamine from histidine via the enzyme histidine decarboxylase. Elevated levels of bacterial fermentation can lead to elevated histamine in raw meat and food products. Histamine levels thus can be used as an indicator of spoilage.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Histamine Enzyme Mix 1 vial Histamine Probe 1 vial Histamine Standard (50 mM) 1 x 100µl Histamine Assay Buffer 1 x 25ml -
Research areas
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Relevance
Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. -
Cellular localization
Secreted -
Alternative names
- 2 (4 imidazolyl)ethylamine
- 4 (2 aminoethyl) 1 3 diazole
Images
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Example Data
Histamine Standard Curve.
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Estimation of Histamine concentration in putrid fish and fish sauce.Fish sauce was deproteinized using 10 kDa spin colum and diluted 20-fold using Histamine Assay Buffer. Histamine concentrations are: Putrid Fish: 88 ± 8 ppm, Fish Sauce: 847 ± 27 ppm.
