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Rat Arginase1 ELISA Kit (ab231923)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 22.1 pg/ml
  • Range: 156.25 pg/ml - 10000 pg/ml
  • Sample type: Tissue Lysate
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat Arginase1 ELISA Kit
    See all Liver Arginase kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Liver lysate 8 3.2%
    Inter-assay
    Sample n Mean SD CV%
    Liver lysate 3 4.1%

  • Sample type

    Tissue Lysate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    22.1 pg/ml

  • Range

    156.25 pg/ml - 10000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Lysate 98 96% - 102%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Rat Arginase1 ELISA Kit (ab231923) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Arginase1 protein in tissue lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Arginase1 with 22.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat Arginase 1 Capture Antibody 1 x 600µl
    10X Rat Arginase 1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    Plate Seals 1 unit
    Rat Arginase 1 Lyophilized Recombinant Protein 2 vials
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.

  • Involvement in disease

    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.

  • Sequence similarities

    Belongs to the arginase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • A I
    • Al
    • ARG 1
    • arg1
    • ARGI1_HUMAN
    • Arginase 1
    • Arginase liver
    • Arginase type I
    • Arginase, liver
    • Arginase-1
    • Arginase1
    • Liver type arginase
    • Liver-type arginase
    • Type I arginase
    see all

  • Database links

    Images

    • Other - Rat Arginase1 ELISA Kit (ab231923)
      Other - Rat Arginase1 ELISA Kit (ab231923)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of rat Arginase1 standard curve in 1X Cell Extraction Buffer PTR
      Example of rat Arginase1 standard curve in 1X Cell Extraction Buffer PTR

      The Arginase1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Arginase1 in rat liver lysate based on a 2.5 µg/mL extract load
      Interpolated concentrations of native Arginase1 in rat liver lysate based on a 2.5 µg/mL extract load

      The concentrations of Arginase1 were measured in duplicate and interpolated from the Arginase1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Arginase1 concentration was determined to be 1819.14 pg/mL in liver lysate.

    • Sandwich ELISA - Rat Arginase1 ELISA Kit (ab231923)
      Sandwich ELISA - Rat Arginase1 ELISA Kit (ab231923)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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