Ornithine Assay Kit (Fluorometric) (ab252903)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Other biological fluids, Serum, Tissue
Overview
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Product name
Ornithine Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Other biological fluids, Tissue -
Assay type
Quantitative -
Product overview
The Ornithine Assay Kit (Fluorometric) (ab252903) provides a rapid, specific, and easy method for the measurement of total ornithine concentrations in a wide variety of biological samples. In this enzyme-based assay, ornithine is converted into a series of intermediates, which will further react with a probe producing a stable fluorometric signal (Ex/Em = 535/587nm). The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 50 pmol/well of ornithine in biological samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Ornithine Assay Buffer 1 x 25ml Ornithine Converter Mix 1 vial Ornithine Developer Mix 1 vial Ornithine Enzyme Mix 1 vial Ornithine Probe (in DMSO) 1 x 220µl Ornithine Standard 1 vial Tissue Cleanup Mix 1 vial -
Research areas
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Relevance
Ornithine is one of the products of the action of the enzyme arginase on L-arginine, creating urea. Therefore, ornithine is a central part of the urea cycle, which allows for the disposal of excess nitrogen.
Images
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Ornithine Standard curve
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Specificity of the detection of Ornithine over other amino acids.Specificity of the detection of Ornithine over other amino acids. Other amino acids were tested at a 10-fold molar excess (each aa: 10nmol) vs Ornithine (1 nmol).
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Estimations of Ornithine in human serum sample (10 µL) and rat liver (62 µg protein).Estimations of Ornithine in human serum sample (10 µL) and rat liver (62 µg protein). Ornithine concentrations were 82.1 µM in human serum sample, and 14.5 pmol/µg-protein in rat liver. Assays were perfromed following the kit protocol.
