Rat ANP ELISA Kit (NPPA) (ab108797)
Key features and details
- Sensitivity: 6.7 pg/ml
- Range: 0.008 ng/ml - 2 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat ANP ELISA Kit (NPPA)
See all Natriuretic peptides A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.1% Inter-assay Sample n Mean SD CV% Overall 10.1% -
Sample type
Cell culture supernatant, Serum, Plasma, Tissue -
Assay type
Sandwich (quantitative) -
Sensitivity
= 6.7 pg/ml -
Range
0.008 ng/ml - 2 ng/ml -
Recovery
102 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat
Predicted to work with: Pig
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Product overview
Abcam’s ANP (NPPA) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ANP concentrations in cell culture supernatants, tissue-extract, serum and plasma.
An ANP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an ANP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of ANP captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 60X Biotinylated Rat ANP Antibody 1 x 100µl ANP Microplate (12 x 8 well strips) 1 unit ANP Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Involvement in disease
Atrial standstill 2
Atrial fibrillation, familial, 6 -
Sequence similarities
Belongs to the natriuretic peptide family. -
Post-translational
modificationsCleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANF
- ANF_HUMAN
- ANP
see all -
Database links
- Entrez Gene: 397496 Pig
- Entrez Gene: 24602 Rat
- SwissProt: P24259 Pig
- SwissProt: P01161 Rat
- Unigene: 2004 Rat
Images
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Sandwich ELISA - ANP (NPPA) Rat ELISA kit (ab108797)Rat ANP measured in biological fluids, background signal subtracted (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab108797.