Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin) (ab272184)
![Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin) (ab272184)](https://www.abcam.com/ps/products/272/ab272184/Images/ab272184-385444-recombinant-Biotin-AntiHuman-IgG2-rabbit-monoclonal-antibody-RM118-titerelisa-human.png "Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin) (ab272184)")
Key features and details
- Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA
Overview
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Product name
Rabbit monoclonal [RM118] Anti-Human IgG2 heavy chain (Biotin) -
Host species
Rabbit -
Target species
Human -
Specificity
ab272184 reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
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Tested applications
Suitable for: ELISAmore details -
Immunogen
Full length protein corresponding to Human IgG2.
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
From an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM118 -
Isotype
IgG -
Research areas
Images
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ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)
A titer ELISA using ab272184. The plate was coated with different amounts of human IgG2. A serial dilution of ab272184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)ELISA of human immunoglobulins shows ab272184 only reacted to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of ab272184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)Sandwich ELISA using ab272184 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
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Sandwich ELISA - Rabbit monoclonal RM118 Anti-Human IgG heavy chain (Biotin) (ab272184)Sandwich ELISA using ab272184 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.