Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)
![Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)](https://www.abcam.com/ps/products/238/ab238623/Images/ab238623-339430-ab2386232.jpg "Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)")
Key features and details
- Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA
Overview
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Product name
Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin)
See all IgG1 secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Specificity
ab238623 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
The Fc region of ab238623 has been engineered to eliminate Fc receptor binding. -
Tested applications
Suitable for: WB, ELISAmore details -
Immunogen
Full length protein corresponding to Mouse IgG1. (Mouse IgG).
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM106 -
Isotype
IgG -
Research areas
Images
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Western blot - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)All lanes : Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623) at 0.2 µg/ml
Lane 1 : Non-reduced (-) mouse IgG1, 20 ng/lane
Lane 2 : Reduced (+) mouse IgG1, 20 ng/lane
Predicted band size: 36 kDaThis antibody reacts to non-reduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
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![ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)](https://www.abcam.com/ps/products/238/ab238623/Images/ab238623-339429-ab2386233.jpg)
ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)](https://www.abcam.com/ps/products/238/ab238623/Images/ab238623-319897-ab238623.jpg)
ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)ELISA of mouse immunoglobulins shows ab238623 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.