Human Anti-Rubella virus IgG ELISA Kit (ab108767)
Key features and details
- Sensitivity: 0.45 U/ml
- Range: 10 U/ml - 100 U/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-Rubella virus IgG ELISA Kit
See all Rubella Virus IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Standard B 6 7.9% Standard C 6 3.4% Standard D 6 2.5% Inter-assay Sample n Mean SD CV% Standard B 8 7.6% Standard C 6 4.6% Standard D 6 6.3% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
0.45 U/ml -
Range
10 U/ml - 100 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Rubella virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Rubella virus in Human serum and plasma.
A 96-well plate has been precoated with Rubella virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Rubella virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rubella virus IgG sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Rubella virus (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Rubella virus anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Rubella virus IgG Standard A – 0 U/mL Yellow, Blue cap 1 x 2ml Rubella virus IgG Standard B – 10 U/mL Yellow, Green cap 1 x 2ml Rubella virus IgG Standard C – 50 U/mL Yellow, Yellow cap 1 x 2ml Rubella virus IgG Standard D – 100 U/mL Yellow, Red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative Standard Curve using ab108767