Anti-Rubella Virus IgM ELISA Kit (ab178654)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
Overview
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Product name
Anti-Rubella Virus IgM ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos Serum 23 2.7% Pos Serum 24 4% Inter-assay Sample n Mean SD CV% Pos. Serum 3 10.3% Pos. Serum 3 11.5% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (qualitative) -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s anti-Rubella Virus IgM in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Rubella Virus in Human serum and plasma.
A 96-well plate has been precoated with anti-Human IgM antibodies to bind to corresponding antibodies of the sample. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Rubella Virus antigen conjugate is added to the wells, which binds to the captured Rubella Virus-specific IgM antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rubella Virus IgM sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Anti-Human IgM Coated Microplate (12 x 8 wells) 1 unit Cover Foil 1 unit IgM Sample Diluent 1 x 100ml Rubella Virus HRP Conjugate 1 x 12ml Rubella Virus IgM Cut-off Control 1 x 3ml Rubella Virus IgM Negative Control 1 x 2ml Rubella Virus IgM Positive Control 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
