Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, ELISA, IHC-P, WB
Overview
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Product name
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, ELISA, IHC-P, WBmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Conjugation notes
Molar enzyme: antibody protein ratio is 1:1 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Images
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Western blot - Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/5000 dilution
Lanes 3-4 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/20000 dilution
Lanes 5-6 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 minutes