ASK1 overexpression 293T lysate (whole cell) (ab94062)
Overview
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Product name
ASK1 overexpression 293T lysate (whole cell) -
General notes
ab94062 is a 293T cell transfected lysate in which Human ASK1 has been transiently over-expressed using a pCMV-ASK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. Tissue specificity: Abundantly expressed in heart and pancreas. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. PTM: Dephosphorylated and activated by PGAM5.
Images
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SDS-PAGE - ASK1 overexpression 293T lysate (whole cell) (ab94062)ab94062 at 15µg/lane on an SDS-PAGE gel.
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Western blot - ASK1 overexpression 293T lysate (whole cell) (ab94062)
