Pyridoxal 5'-phosphate (Vit B6) Assay Kit (Fluorometric) (ab273312)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Other biological fluids, Tissue
Overview
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Product name
Pyridoxal 5'-phosphate (Vit B6) Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Other biological fluids, Tissue -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Pyridoxal 5’-phosphate (Vit B6) Assay Kit (Fluorometric) (ab273312) provides a quick, specific and easy method for measuring Pyridoxal 5’-phosphate (PLP) concentrations in a wide variety of samples.
In this assay, PLP reacts with a PLP-dependent enzyme thereby converting the substrate into an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 0.2 pmol/well of PLP in biological samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests PLP Assay Buffer 1 x 25ml PLP Developer 1 (Lyophilized) 1 vial PLP Developer 2 (Lyophilized) 1 vial PLP Enzyme Mix (Lyophilized) 1 vial PLP Probe 1 x 200µl PLP Standard (Lyophilized) 1 vial PLP Substrate (Lyophilized) 1 vial -
Research areas
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Relevance
Vitamin B6 is a water-soluble vitamin that exists in three major chemical forms: pyridoxine, pyridoxal and pyridoxamine. Vitamin B6 is needed for more than 100 enzymes involved in protein metabolism. It is also essential for red blood cell metabolism. The nervous and immune systems need Vitamin B6 to function efficiently and it is also needed for the conversion of tryptophan to niacin. -
Alternative names
- Pyridoxal
- Pyridoxamine
- Pyridoxine
Images
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PLP standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example data -
Example dataExample data.
Estimation of PLP in orange juice (5 µL of 100X dilution), human serum (4 µL) and rat liver lysate (5 µL of 10X dilution). PLP concentrations were 4.36± 0.40 µM in orange juice, 15.98 ± 0.58 nM in human serum and 79.0 ± 4.0 pmol/mg in rat liver lysate samples. Assays were performed following the kit protocol.