Proteinase K Antigen Retrieval Solution (ab64220)
Overview
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Product name
Proteinase K Antigen Retrieval Solution -
Tested applications
Suitable for: IHC-P, In situ hybridizationmore details -
General notes
ab64220 is a single-component, ready-to-use proteinase K solution for use in protease-induced antigen retrieval during IHC (PIER) and pre-treatment for in situ hybridization.
Staining Protocol:
- IHC procedure: Incubate tissue section for 5 to 7 minutes at room temperature; Rinse and proceed with immunostaining.
- General ISH procedure: Incubate tissue section for 15 minutes at 37oC; Rinse and proceed with hybridization.
Other products for IHC
Other enzymes for antigen retrieval include: Pepsin Solution ab64201 and Trypsin Antigen Retrieval Kit ab259256.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
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Form
Liquid -
Storage instructions
Store at -20°C. Stable for 12 months at -20°C -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: Tris buffer, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Relevance
Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes. -
Alternative names
- Endopeptidase K
- Tritirachium alkaline proteinase
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K (ab64220)ab64220 Proteinase K used with aa anti-Pan CK antibody on Human skin