Protein G Agarose (High Affinity) (ab193258)
Key features and details
- Sample type: Ascites Fluid, Cell culture media, Cell culture supernatant, Serum
Overview
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Product name
Protein G Agarose (High Affinity) -
Sample type
Cell culture supernatant, Serum, Cell culture media, Ascites Fluid -
Product overview
Very high binding capacity (>30 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG, immunoprecipitation & ChIP (ab193258).
Contents:
Supplied as 50% slurry in 20% Ethanol.
Features:
High binding capacity: Binding of IgG ≥ 30 mg human or rabbit IgG/mL Protein G Agarose.
Minimal leaching of the ligand
Flow Rate Tested*: 2.89 mL/min.
*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Maximum Flow Rate**: 1800 cm/hr; minimum leaching of recombinant Protein G.
**NOTE: the highest flow that beads withstand for 1 minute, without collapsing and the pressure reaching 1 MPa.Usage:Reusable for up to 10 times without significant loss of binding capacity.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. -
Notes
Protein G Agarose (High Affinity) beads are specially prepared for high IgG binding by covalently coupling recombinant Protein G to 6% cross-linked agarose beads, the most popular resin for protein affinity purification methods. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein G than standard Protein G agarose beads. The IgG binding capacity of Protein G Agarose (High Affinity) is ≥ 30 mg of human or rabbit IgG per mL of wet beads. The Protein G Agarose (High Affinity) beads display high chemical and physical stability, as well as high flow rate, hydrophilicity and high gel strength.
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Tested applications
Suitable for: IP, Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml 25 ml 100 ml Hi-Bind™ Protein G-Agarose 1 x 1ml 1 x 5ml 1 x 25ml 1 x 100ml -
Research areas
