Protein A/G/L Sepharose® Column (ab193265)
Overview
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Product name
Protein A/G/L Sepharose® Column -
Product overview
Contents:
Supplied as ready-to-use pre-packed columns of 1 mL or 5 mL bead volume in PBS with 0.02% sodium azide.
Features:
Protein A/G/L binds to all IgG subclasses from various mammalian species that bind to Protein A, Protein G, and Protein L, making it the ideal choice for purification of a wide range of polyclonal or monoclonal IgG antibodies. The binding capacity is greater than 10 mg/mL of bead; high flow rate; low falling off of rProtein A/G/L; pH stability 2-10.
Store column at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of monoclonal and polyclonal antibodies.
Sepharose is a registered trademark of GE Healthcare
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Notes
Protein A/G/L-Sepharose® is prepared by covalently coupling recombinant Protein A/G/L (containing five Ig-binding regions from Protein L, five Ig-binding domains from Protein A, and three Ig-binding regions from Protein G) to 6% cross-linked Sepharose® beads. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure maximum specific IgG binding. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet bead.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml Protein A/G/L Sepharose® Column 1 x 1ml 1 x 5ml -
Research areas
