PP3, Negative control for PP2 (ab120617)
Key features and details
- Negative control for the Src kinase inhibitor PP2.
- CAS Number: 5334-30-5
- Purity: > 97%
- Soluble in DMSO to 100 mM and in ethanol to 25 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
PP3, Negative control for PP2 -
Description
Negative control for the Src kinase inhibitor PP2. -
Biological description
Negative control for the Src kinase inhibitor PP2 (ab120308). Inhibits EGFR kinase (IC50 = 2.7 μM).
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Purity
> 97% -
CAS Number
5334-30-5 -
Chemical structure
Properties
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Chemical name
4-Amino-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine -
Molecular weight
211.23 -
Molecular formula
C11H9N5 -
PubChem identifier
4879 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 25 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=CC=C(C=C1)N2C3=C(C=N2)C(=NC=N3)N -
Source
Synthetic
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Research areas
Images
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Functional Studies - PP3, Negative control for PP2 (ab120617)ab11512 staining E cadherin in SW480 cells treated with PP3 (ab120617), by ICC/IF. No change of E cadherin expression with increased concentration of PP3 is observed, as described in literature, since PP3 is the negative control for PP2.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120617 (PP3) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.