Potassium Assay Kit (Fluorometric) (ab252904)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Serum
- Sensitivity: 10 nmol/well
Overview
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Product name
Potassium Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum -
Assay type
Quantitative -
Sensitivity
10 nmol/well -
Product overview
The Potassium Assay Kit (Fluorometric) (ab252904) is a simple plate based assay kit for the measurement of potassium concentration in human serum samples. It is based on a potassium detection agent that is activated by potassium. The detection agent catalyzes a reaction between two substrates in presence of potassium, leading to the formation of an intermediate, which further reacts with the developer and oxidizes a non-fluorescent probe producing a strong, stable fluorescence signal.
The rate of the reaction is proportional to the potassium concentration present in the sample and can be monitored at Ex/Em= 535/587 nm. Sodium concentrations of up to 10 fold that of potassium, do not interfere with the assay. However, since serum has about 25-30 times higher sodium levels that potassium, it can slightly activate the potassium detection agent (although to a much lower extent than potassium).
A sodium masking agent has been included in our kit, which prevents any activation caused due to sodium present in serum delivering highly a specific assay for the detection of potassium. The kit can detect as low as 10 nmol of potassium per well and is linear up to 50 nmol.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests K Assay Buffer 1 x 25ml K Detection Solution 1 vial K Developer 1 vial K Probe 1 x 200µl K Standard (5 mM) 1 x 500µl K Substrate I 1 vial K Substrate II 1 vial Sodium Masking Solution 1 x 500µl
Images
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Potassium Standard Curve
Potassium standard curve.
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Estimated potassium concentrations in “off-the-clot” human serumEstimated potassium concentrations in “off-the-clot” human serum. Spiked experiments show 99 ± 5 % recovery.
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Reaction kinetics.Reaction kinetics for 100 µM (10 nmol) potassium standard and for 2.5 µl “off-the-clot” human serum
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Sodium concentrations of up to 10 fold that of potassium do not interfere with the assay.Sodium concentrations of up to 10 fold that of potassium do not interfere with the assay.