Porcine IL-22 ELISA Kit (ab273212)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 20 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine IL-22 ELISA Kit
See all IL-22 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
20 pg/ml -
Range
20 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 91.41 83% - 97% Plasma 70.97 68% - 81% Cell culture extracts 98.98 88% - 110% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine IL-22 ELISA Kit (ab273212) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-22 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for porcine IL-22 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-22 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine IL-22 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-22 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine IL-22 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine IL-22 Antibody 2 vials Porcine IL-22 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine that contributes to the inflammatory response in vivo. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine Zcyto18
- IL 10 related T cell derived inducible factor
- IL 21
see all
Images
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Example standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.