Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab277777)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal to GSK3 (alpha + beta) (phospho Y216 + Y279)
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody
See all GSK3 (alpha + beta) primary antibodies -
Description
Rabbit recombinant multiclonal to GSK3 (alpha + beta) (phospho Y216 + Y279) -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human GSK3 (alpha + beta) aa 200-300 (phospho Y216 + Y279).
Database link: P49840 -
Positive control
- ICC: MCF-7 cells. WB: 3T3, 3T3-L1 treated with Insulin (100nM-10min), A549, A549 treated with Insulin (100nM-10min) whole cell extract.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSK3 (alpha + beta) antibody (ab277777)All lanes : Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab277777) at 2 µg/ml
Lane 1 : 3T3-L1 whole cell extract
Lane 2 : 3T3-L1 treated with Insulin (100nM-10min) whole cell extract
Lane 3 : A549 whole cell extract
Lane 4 : A549 treated with Insulin (100nM-10min) whole cell extract
Lane 5 : 3T3-L1 whole cell extract with Phosphopeptide at 10 µg/ml
Lane 6 : 3T3-L1 treated with Insulin (100nM-10min) whole cell extract with Phosphopeptide at 10 µg/ml
Lane 7 : A549 whole cell extract with Phosphopeptide at 10 µg/ml
Lane 8 : A549 treated with Insulin (100nM-10min) whole cell extract with Phosphopeptide at 10 µg/ml
Lysates/proteins at 30 µg per lane.
Secondary
Lanes 1-4 : HRP conjugate Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, 0.4 µg/mL at 1/2500 dilution
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Immunocytochemistry - Anti-GSK3 (alpha + beta) antibody (ab277777)For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous GSK-3 alpha (pY279) + GSK3 beta (pY216) using Anti-GSK-3 alpha (pY279) + GSK3 beta (pY216) Recombinant Rabbit Multiclonal Antibody (ab277777, 2 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of GSK-3 alpha (pY279) + GSK3 beta (pY216) protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear and cytoplasmic localization of GSK-3 alpha (pY279) + GSK3 beta (pY216). Antibody specificity was demonstrated by competition with the GSK-3 alpha (pY279) + GSK3 beta (pY216) phospho peptide, which results in loss of signal in panel e). Panel f) shows no competition with the non-phospho peptide. The images were captured at 60X magnification
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Anti-GSK3 (alpha + beta) antibody (ab277777)