Porcine IL-1 alpha ELISA Kit (ab273264)
Key features and details
- Sensitivity: 4.5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine IL-1 alpha ELISA Kit
See all IL-1 alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.5 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103.2 77% - 124% Plasma 90.32 70% - 117% Cell culture media 125.9 98% - 139% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine IL-1 alpha ELISA Kit (ab273264) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IL-1 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Porcine IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests Porcine IL-1 alpha Standard (Lyophilized) 2 vials 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Anti- Porcine IL-1 alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti- Porcine IL-1 alpha Antibody 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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Alternative names
- BAF
- FAF
- Hematopoietin 1
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Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.