Porcine Decorin ELISA Kit (ab273221)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Pig
Overview
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Product name
Porcine Decorin ELISA Kit
See all Decorin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.43 93% - 104% Serum 116.8 92% - 142% Plasma 93.49 67% - 91% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human, Pig -
Product overview
Porcine Decorin ELISA Kit 9ab273221)) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine Decorin in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as anti-coagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for porcine Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Porcine Decorin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine Decorin Antibody 2 vials Porcine Decorin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
May affect the rate of fibrils formation. -
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Bone proteoglycan II
- CSCD
- DCN
see all -
Database links
- Entrez Gene: 1634 Human
- Entrez Gene: 396957 Pig
- Omim: 125255 Human
- SwissProt: P07585 Human
- SwissProt: Q9XSD9 Pig
- Unigene: 728830 Human
Images
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Porcine Decorin Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine Decorin ELISA kit (ab273221) Standard curve.
