PKA Kinase Activity Assay Kit (ab139435)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 4 hr 30 min
- Sample type: Adherent cells, Purified protein, Suspension cells, Tissue Extracts
Overview
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Product name
PKA Kinase Activity Assay Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample Inter-assay Sample n Mean SD CV% Sample -
Sample type
Adherent cells, Suspension cells, Tissue Extracts, Purified protein -
Assay type
Enzyme activity -
Assay time
4h 30m -
Product overview
PKA Kinase Activity Kit (ab139435) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of PKA and for quantitating the activity of PKA in purified or partially purified enzyme preparations. This kit is based on a solid phase enzyme-linked immuno-absorbent assay (ELISA) that utilizes a specific synthetic peptide as a substrate for PKA and a polyclonal antibody that recognizes the phosphorylated form of the substrate. The assay is designed for the analysis of PKA activity in the solution phase. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols.
The kit offers the following advantages:
1. Safe - non-radioactive measurement of kinase activity
2. Flexible - kinetic and end-point options available
3. Fast - results in
4. Efficient - only 30 µl diluted sample needed per well
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 30ml Active PKA 20 ng/µl 1 x 30µl Antibody Dilution Buffer 1 x 10ml Anti-Rabbit IgG: HRP Conjugate 1 x 20µl ATP 1 x 2mg Kinase Assay Dilution Buffer 1 x 10ml PKA Phosphospecific Substrate Antibody 1 x 5ml PKA Substrate Microtiter Plate 1 unit Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Relevance
In cell biology, Protein kinase A (PKA) is a family of enzymes whose activity is dependent on cellular levels of cyclic AMP (cAMP). PKA is also known as cAMP-dependent protein kinase. Protein kinase A has several functions in the cell, including regulation of glycogen, sugar, and lipid metabolism.
Images
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Functional Assay: ab139435 PKA Kinase Activity Assay Kit
1e7 3T3-L1 cells were treated for 15 minutes with 1 μM dexamethasone (ab120743) and 1 μg/mL insulin (ab123768) with the addition of 50 μM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424). Cell extracts were prepared according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).
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Functional Assay: ab139435 PKA Kinase Activity Assay KitTitration of positive control (active PKA), background signal subtracted (duplicates; +/- SD).
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Functional Assay: ab139435 PKA Kinase Activity Assay KitTitration of cAMP Protein Kinase (ab56268), background signal subtracted (duplicates; +/- SD).
