Phospholipase D Activity Assay Kit (Colorimetric) (ab183306)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Phospholipase D Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Phospholipase D Activity Assay Kit (Colorimetric) (ab183306) provides a simple and sensitive method of measuring Phospholipase D activity using colorimetry (OD 570 nm). In this assay, Phospholipase D cleaves choline from phosphatidylcholine. The free choline is then oxidized by the Phospholipase D enzyme mix to generate an intermediate which reacts with the Phospholipase D Probe to generate color that is detected at OD= 570 nm. This high-throughput adaptable assay kit can detect as little as 1.0 mU/mL Phospholipase D activity in cells and tissues.
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Notes
Phospholipase D is a key player in phospholipid metabolism. Phospholipase D hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. Phospholipase D activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in Phospholipase D activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Choline Standard (Lyophilized) 1 vial Phospholipase D Assay Buffer 1 x 25ml Phospholipase D Enzyme Mix (Lyophilized) 1 vial Phospholipase D Positive Control (Lyophilized) 1 vial Phospholipase D Probe (in DMSO) 1 x 200µl Phospholipase D Substrate 1 x 100µl -
Relevance
Phospholipase D is a key player in phospholipid metabolism. Phospholipase D hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. Phospholipase D activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in Phospholipase D activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders.
Images
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Phospholipase D Activity Assay Kit (ab183306)
Typical Choline Standard calibration curve.
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Phospholipase D Activity Assay Kit (ab183306)Phospholipase D activity in liver lysate (5 µL) & Positive Control (1 µL).