PE Anti-PD1 antibody [NAT105] (ab220302)
![PE Anti-PD1 antibody [NAT105] (ab220302)](https://www.abcam.com/ps/products/220/ab220302/Images/ab220302-312646-anti-pd1-antibody-nat105-phycoerythrin-flow-cytometry.jpg "PE Anti-PD1 antibody [NAT105] (ab220302)")
Key features and details
- PE Mouse monoclonal [NAT105] to PD1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-PD1 antibody [NAT105]
See all PD1 primary antibodies -
Description
PE Mouse monoclonal [NAT105] to PD1 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Tissue, cells or virus corresponding to Human PD1. YT cells (human T/NK cell Leukemia)
Database link: Q15116 -
Positive control
- Flow Cyt: Molt-4 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
NAT105 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - PE Anti-PD1 antibody [NAT105] (ab220302)
Overlay histogram showing Molt-4 cells stained with ab220302 (red line). The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab220302, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
