Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (ab273328)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (ab273328) provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, Phosphoglycerate Dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal (OD: 450 nm).
The assay is simple, sensitive, high–throughput adaptable and can detect Phosphoglycerate Dehydrogenase activity less than 0.1 mU per sample.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests NADH Standard (Lyophilized) 1 vial PHGDH Assay Buffer 1 x 25ml PHGDH Developer (Lyophilized) 1 vial PHGDH Positive Control 1 x 50µl PHGDH Substrate (Lyophilized) 1 vial -
Research areas
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Pathway
Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3. -
Involvement in disease
Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures. -
Sequence similarities
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. - Information by UniProt
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Alternative names
- 3 PGDH
- 3-PGDH
- 3-phosphoglycerate dehydrogenase
see all
Images
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Example data
Example data.
NADH Standard Curve.
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Example dataExample data.
Kinetic measurement of Phosphoglycerate Dehydrogenase activity from various samples.
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Example dataExample data.
Relative PHGDH Activity was calculated in lysates prepared from HEK cells (4.5µg) and HeLa cells (7.8µg).