PE Anti-IL-2 Receptor alpha antibody [PC61.5] (ab210334)
![PE Anti-IL-2 Receptor alpha antibody [PC61.5] (ab210334)](https://www.abcam.com/ps/products/210/ab210334/Images/ab210334-256733-anti-cd25-antibody-pc615-phycoerythrin-flow-cytometry.jpg "PE Anti-IL-2 Receptor alpha antibody [PC61.5] (ab210334)")
Key features and details
- PE Rat monoclonal [PC61.5] to IL-2 Receptor alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-IL-2 Receptor alpha antibody [PC61.5]
See all IL-2 Receptor alpha primary antibodies -
Description
PE Rat monoclonal [PC61.5] to IL-2 Receptor alpha -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- C57Bl/6 mouse spleen cells
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General notes
ab210334 is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naive or conventional T cells which are CD25-.
The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab210334 was purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
PC61.5 -
Isotype
IgG1 -
Light chain type
lambda -
Research areas
Images
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Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [PC61.5] (ab210334)
Flow cytometric analysis of mouse C57Bl/6 spleen cells stained with APC Anti-Mouse Anti-IL-2 Receptor alpha antibody and ab210334 at 0.125 µg (right panel), or PE Rat IgG1 isotype control 0.125 µg (left panel).
