PE Anti-CD4 antibody [EPR20122] (ab252151)
![PE Anti-CD4 antibody [EPR20122] (ab252151)](https://www.abcam.com/ps/products/252/ab252151/Images/ab252151-1-RabbitmonoclonalEPR20122toCD4RPEmousesplenocytescellsflowcytometry.jpg "PE Anti-CD4 antibody [EPR20122] (ab252151)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR20122] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-CD4 antibody [EPR20122]
See all CD4 primary antibodies -
Description
PE Rabbit monoclonal [EPR20122] to CD4 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cytometry: C57 BL/6 mouse splenocytes.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20122 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - PE Anti-CD4 antibody [EPR20122] (ab252151)
C57 BL/6 mouse splenocytes stained with ab252151 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/CD32 and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab252151) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (1x 106 in 100 µl at 1 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD8.
Acquisition of >30,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. Events were gated on live CD3 positive T cells.
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![PE Anti-CD4 antibody [EPR20122] (ab252151)](https://www.abcam.com/ps/products/252/ab252151/Images/ab252151-2-benefits-of-recombinant-antibodies.png)
PE Anti-CD4 antibody [EPR20122] (ab252151)