Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14902] to PSMB10/MECL1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PSMB10/MECL1 antibody [EPR14902]
    See all PSMB10/MECL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14902] to PSMB10/MECL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Daudi, HuT 78 and Raji cell lysates; Human fetal spleen and thymus tissue lysates. IHC-P: Human kidney adenocarcinoma tissue. Flow Cyt: Daudi cells.

  • General notes

    This product was previously labelled as PSMB10.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PSMB10 knockout HeLa cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab183506 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab183506 Anti-PSMB10/MECL1 antibody [EPR14902] was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264923 (knockout cell lysate ab258145) was used.  Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE.  ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

    Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

    Flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution

    Lane 1 : Human fetal spleen tissue lysate
    Lane 2 : Human fetal thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 29 kDa

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/5000 dilution

    Lane 1 : HuT 78 cell line lysate
    Lane 2 : Raji cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 29 kDa

  • Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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