pan Caspase (active) Red Staining Kit (ab65616)
Key features and details
- Assay type: Enzyme activity
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Assay time: 2 hr
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
pan Caspase (active) Red Staining Kit
See all Caspase kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Enzyme activity -
Assay time
2h 00m -
Product overview
Abcam's pan Caspase (active) Red Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes the caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent in situ marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
Visit our FAQs page for tips and troubleshooting. -
Notes
Activation of caspases plays a central role in apoptosis.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
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Platform
Microplate reader, Fluor. microscope, Flow cyt.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Red-VAD-FMK 1 x 100µl Wash Buffer 2 x 100ml Z-VAD-FMK 1 x 10µl -
Research areas
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Relevance
Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. -
Cellular localization
Cytoplasmic
