Oxalate Decarboxylase Activity Assay Kit (Colorimetric) (ab196993)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Tissue
Overview
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Product name
Oxalate Decarboxylase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Plants, Fungi -
Product overview
Oxalate Decarboxylase Activity Assay Kit (Colorimetric) (ab196993) provides a quick and easy way to measure oxalate decarboxylase (OXDC) activity in a variety of plant and fungi samples. Oxalate is converted by OXDC to formate, which in turn converts a nearly colorless probe to a colored product with strong absorbance at OD=450 nm. The sensitivity of this assay is less than 20 μU of OXDC activity.
The assay is simple, sensitive, high-throughput adaptable.
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Notes
Oxalate Decarboxylase (OXDC, EC 4.1.1.2) belongs to the cupin superfamily and is composed of two β-barrel domains. OXDC catalyzes the conversion of Oxalate into Formate and CO2 and plays an important role in stress response. In humans, high levels of oxalate can lead to various health problems including hyperoxaluria, kidney stones, and renal failure. Wood rotting fungi generates high levels of oxalate, causing rot in many crops including lettuce, soybean, dry bean, and tomato etc. Recent studies show that overexpression of OXDC in plants (e.g. tomato, soybean, lettuce, and tobacco) results in transgenic plants with resistance to fungal pathogenesis. Accurate measurement of Oxalate Decarboxylase activity is useful for a variety of therapeutic, diagnostic, and mechanistic studies.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Formate Standard (100 mM) Yellow cap 1 x 0.1ml OXDC Assay Buffer I 1 x 20ml OXDC Assay Buffer II 1 x 15ml OXDC Enzyme Mix 1 vial OXDC Probe 1 vial OXDC Substrate 1 vial Positive Control 1 x 0.1ml
Images
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Oxalate Decarboxylase Activity Assay Kit (Colorimetric) (ab196993)
Oxalate Decarboxylase (OXDC) activity was measured in white mushroom lysate (20 μL), and Jade Plant lysate (20 μL).
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Oxalate Decarboxylase Activity Assay Kit (Colorimetric) (ab196993)TYpical Formate Standard Curve.