Human TNFRSF14 ELISA Kit (ab216950)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.2 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human TNFRSF14 ELISA Kit
See all TNFRSF14/HVEM kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 5 2.9% Inter-assay Sample n Mean SD CV% Human serum 3 4.5% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
9.2 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107 103% - 113% Cell culture extracts 105 100% - 109% Tissue Extracts 105 103% - 106% Cell culture media 116 114% - 119% Hep Plasma 102 98% - 105% EDTA Plasma 102 91% - 110% Cit plasma 107 100% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human TNFRSF14 ELISA Kit (ab216950) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNFRSF14 protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TNFRSF14 with 9.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
TNFRSF14 (also known as tumor necrosis factor receptor superfamily member 14 or Herpesvirus entry mediator or HVEM) is a cell surface receptor of the TNF-receptor superfamily. TNFRSF14 is a receptor for BTLA, LIGHT, lymphotaxin alpha. Herpesvirus glycoprotein D binds to TNFRSF14.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TNFRSF14 Capture Antibody 1 x 600µl 10X Human TNFRSF14 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human TNFRSF14 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. -
Tissue specificity
Widely expressed, with the highest expression in lung, spleen and thymus. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
-
Alternative names
- HVEML
- ATAR
- CD270
see all -
Database links
- Entrez Gene: 8764 Human
- Omim: 602746 Human
- SwissProt: Q92956 Human
- Unigene: 512898 Human
Images
-
Other - Human TNFRSF14 ELISA Kit (ab216950)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human TNFRSF14 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Example of human TNFRSF14 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native TNFRSF14 in human serum and plasma samples.The concentrations of TNFRSF14 were measured in duplicates, interpolated from the TNFRSF14 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 2,507 pg/mL in serum, 2,050 pg/mL in plasma (citrate) and 1,696 pg/mL in plasma (EDTA) and 2,044 pg/mL in plasma (heparin).
-
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 2413 pg/mL with a range of 1201 – 4953 pg/mL.
-
Comparison of secreted TNFSF14 in unstimulated and PMA/PHA-stimulated Peripheral Blood Monocyte (PBMC) Cells.PBMC cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. TNFRSF14 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated PBMC and cell culture media. Measured values were interpolated from the TNFRSF14 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 211 pg/mL in unstimulated, 744 pg/mL in stimulated, and undetectable in media.
-
Interpolated concentrations of native TNFRSF14 in human Raji and HL-60 cell extracts and human liver homogenate tissue extract samples.Samples based on a 400 μg/mL extract load. The concentrations of TNFRSF14 were measured in duplicate and interpolated from the TNFRSF14 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 306 pg/mL in Raji Extract, 345 pg/mL in HL-60 extract, and 809 pg/mL in Human Liver Homogenate Extract.
-
Sandwich ELISA - Human TNFRSF14 ELISA Kit (ab216950)To learn more about the advantages of recombinant antibodies see here.
