OliGlo™ Cy3 FISH Kit (ab253402)
Overview
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Product name
OliGlo™ Cy3 FISH Kit -
Product overview
Fluorescence in situ hybridization (FISH) kit which directly labels the phosphate groups (terminal and backbone) on DNA using a Cy3 labeling reagent prepared in situ, causing less disruption of hybridization and allowing labeling that is not sequence dependent.
This kit when used with the supplied standard labeling protocol will yield labeling efficiency of approximately 10 to 100 labels per kilobase of nucleic acid. This standard size kit is suitable for labeling up to 120 μg of nucleic acid. The trial size kit is suitable for labeling up to 35 μg of nucleic acid.
This kit is sold by Marker Gene under product code M1622/M1623.
Properties
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Storage instructions
Please refer to protocols. -
Components 120 µg 35 µg 3M Sodium Acetate 1 x 500µl 1 x 100µl Activating Resin 1 vial 1 vial Denaturation Buffer D 1 x 200µl 1 x 50µl DMF 1 x 200µl 1 x 50µl Hybridization Buffer H 1 x 750µl 1 x 150µl OliGlo™ Cy3 Labeling Dye Precursor 1 vial 1 vial Renaturation Buffer R 1 x 200µl 1 x 50µl TE Buffer 1 x 500µl 1 x 100µl