Anti-RNF125 antibody (ab74373)
Key features and details
- Rabbit polyclonal to RNF125
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF125 antibody
See all RNF125 primary antibodies -
Description
Rabbit polyclonal to RNF125 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide derived from an internal region of human RNF125.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNF125 antibody (ab74373) This image is courtesy of an anonymous AbreviewAll lanes : Anti-RNF125 antibody (ab74373) at 1/200 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Mouse ES (129Sv) whole cell lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 100 kDa (possible non-specific binding)
Exposure time: 1 minute
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Western blot - Anti-RNF125 antibody (ab74373)All lanes : Anti-RNF125 antibody (ab74373) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from HT-29 cells
Lane 3 : Extracts from K562 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 100 kDa, 23 kDa. We are unsure as to the identity of these extra bands.
