Anti-RNF31/HOIP antibody (ab125189)
Key features and details
- Rabbit polyclonal to RNF31/HOIP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF31/HOIP antibody
See all RNF31/HOIP primary antibodies -
Description
Rabbit polyclonal to RNF31/HOIP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 1022-1072 of Human RNF31/HOIP (NP_060469.4).
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Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNF31/HOIP antibody (ab125189)All lanes : Anti-RNF31/HOIP antibody (ab125189) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-RNF31/HOIP antibody (ab125189)Detection of human RNF31/HOIP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF31/HOIP antibody ab125189 used for IP at 6 µg per reaction. RNF31/HOIP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated RNF31/HOIP, ab125189 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
