Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)]

  • Description

    Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MURF1 aa 1-100. The exact sequence is proprietary.
    Database link: Q969Q1

  • Positive control

    • Rat muscle, Mouse muscle and Human skeletal muscle tissue lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate at 15 µg
    Lane 2 : Mouse muscle lysate at 20 µg

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

    Lane 1 : Rat muscle tissue lysate
    Lane 2 : Mouse muscle tissue lysate
    Lane 3 : Human skeletal muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF3 (full length) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/500 dilution + His-Tagged human MURF2 (full length) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 150 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/5000 dilution + Mouse muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 150 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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