Novel Coronavirus IgM Antibody Detection Kit (SARS-CoV-2) (ab272244)
Overview
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Product name
Novel Coronavirus IgM Antibody Detection Kit (SARS-CoV-2) -
Sample type
Serum, Plasma, Whole Blood -
Species reactivity
Reacts with: Human -
Product overview
Novel Coronavirus IgM Antibody Detection Kit (SARS-CoV-2) (ab272244) is suitable for the qualitative detection of Novel Coronavirus (SARS-CoV-2) IgM antibodies in human serum, plasma, and whole blood.
Note: Novel Coronavirus IgG Antibody Detection Kit (SARS-CoV-2) - ab272243 and Novel Coronavirus IgM Antibody Detection Kit (SARS-CoV-2) - ab272244 are intended for Research Use Only, not for use in diagnostic procedures.
The detection kit uses the principle of immunochromatography: the separation of components in a mixture through a medium using capillary force and the specific and rapid binding of an antibody to its antigen. Each cassette is a dry medium that has been coated separately with novel coronavirus N protein (“T” test line) and goat antichicken IgY antibody (“C” control line) (Figure 1). Two free colloidal gold-labeled antibodies, mouse anti-human IgM (mIgM) and chicken IgY, are in the release pad section (S). Once diluted serum, plasma, or whole blood is applied to the release pad section, the mIgM antibody will bind to coronavirus IgM antibodies if they are present, forming an IgM-IgM complex. The sample and antibodies will then move across the cassette’s medium via capillary action. If coronavirus IgM antibody is present in the sample, the test line (T) will be bound by the IgM-IgM complex and develop color. If there is no coronavirus IgM antibody in the sample, free mIgM will not bind to the test line (T) and no color will develop. The free chicken IgY antibody will bind to the control line (C); this control line should be visible after the detection step as this confirms that the kit is working properly.
The following Detection Kits are available:
Novel Coronavirus IgG Antibody Detection Kit (SARS-CoV-2) - ab272243
Novel Coronavirus IgM Antibody Detection Kit (SARS-CoV-2) - this kit
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Notes
ab272243 and ab272244 were tested on confirmed positive Covid-19 samples and known negative samples. The following data set was the outcome of two different screening batches compiled together. Confirmed positive and negative control sampless were tested for the presence of IgG and IgM novel coronavirus antibodies. The kit was used as instructed by the manual, and positive or negative indications were listed as such. The results of that testing indicates a sensitivity to viral antibodies of 87%, with a specificity of 84%.Sample Type Sample IgM- Ab272244 IgG -Ab272243 Overall Positive Overall Negative Sensitivity Specificity Positive 30 24 24 26 4 87% Negative 90 12 3 14 76 84
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 20 tests Detection Cassette 20 units Dilution Buffer 20 vials -
Research areas
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Relevance
Coronavirus specific IgM -
Alternative names
- COVID-19
- SARS-CoV-2
Images
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Representative test results
Positive for Coronavirus Both the test line (T) and the quality control line (C) are colored dark pink.
Negative for Coronavirus: The test line (T) does not develop color, or a faint gray band may be visible, but the quality control line (C) is colored.
Suspect: A light pink band is an inconclusive result. The sample requires an alternate testing method (such as RT-qPCR) to determine positivity.
Invalid: There is no colored control line (C) band. The results are invalid regardless of whether a red band appears on the test line (T); additional testing is required.