Nitrocefin, Chromogenic cephalosporin beta-lactamase substrate (ab145625)
Key features and details
- Chromogenic cephalosporin β-lactamase substrate
- CAS Number: 41906-86-9
- Purity: > 95%
- Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Nitrocefin, Chromogenic cephalosporin beta-lactamase substrate -
Description
Chromogenic cephalosporin β-lactamase substrate -
Biological description
Chromogenic cephalosporin β-lactamase substrate. Indicates β-lactamase activity via a red color intensely proportional to the original concentration of Nitrocefin. Undergoes rapid distinctive color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm) as the amide bond in the beta-lactam ring is hydrolyzed. -
Purity
> 95% -
CAS Number
41906-86-9 -
Chemical structure
Properties
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Chemical name
(6R,7R)-3-[(E)-2-(2,4-Dinitrophenyl)ethenyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid -
Molecular weight
516.50 -
Molecular formula
C21H16N4O8S2 -
PubChem identifier
6436140 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1C(=C(N2[C@H](S1)[C@@H](C2=O)NC(=O)CC3=CC=CS3)C(=O)O)/C=C/C4=C(C=C(C=C4)[N+](=O)[O-])[N+](=O)[O-] -
Source
Synthetic
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Research areas
