Nile Red Staining Kit (ab228553)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Nile Red Staining Kit -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Product overview
Nile Red Staining Kit ab228553 is designed for the quantitative, fluorometric detection and measurement of intracellular lipid droplets using fluorescence microscopy, flow cytometry or a fluorescence microplate reader.
In the assay, Nile Red dye is added to the cell samples and the dye selectively stains lipid droplets within the cells. The dye is minimally fluorescent in solution in water so it is not necessary to wash the cells or remove the dye before analysis.
Excitation / emission: 550 / 640 nm.
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Notes
Lipid droplets are also referred to as lipid bodies, oil bodies, and adiposomes.
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Platform
Microplate reader, Fluor. microscope, Flow cyt.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests 500 tests Nile Red 1 x 40µl 1 x 40µl Staining Buffer 1 x 20ml 1 x 20ml
Images
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Fluorescence images of intracellular lipid droplets
Fluorescence images of intracellular lipid droplets in 3T3-L1 fibroblasts (left panel) and 3T3-L1 adipocyte cells (right panel) using ab228553 Nile Red Staining Kit. The fluorescence signal was measured using a fluorescence microscope with a TRITC filter.